other:Article Title: A Tac1-Expressing Brainstem Pathway Underlies the Pathogenesis of Trigeminal Neuralgia.
Article Snippet: Briefly, mice were anesthetized with isoflurane (RWD Life Science) and perfused with ice-cold N-methyl-d-glucamine (NMDG) based cutting solution containing (in mM): 93 NMDG, 93 HCl, 30 NaHCO3 , 20 HEPES, 2.5 KCl, 1.2 NaH2 PO4 , 10 MgCl2 , 0.5 CaCl2 , 25 glucose, 3 myo-inositol, 1 sodium-L-ascorbate, 5 Advanced Science, 2026 om m ons L icense TABLE 1 Materials used in this study.
Virus:Article Title: Transient cAMP production drives rapid and sustained spiking in brainstem parabrachial neurons to suppress feeding.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV2/1.hSyn.DIO.GreenDownwardcADDis.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.EF1a.DIO.mKate2-biPAC.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.EF1a.DIO.mKate2-PDE4D3- Cat.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.hSyn.FLEX.GCaMP6s.WPRE Chen et al.59 Addgene Cat# 100845 AAV2/9.hSyn.FLEX.ExRai-AKAR2-CW3SL Richard Huganir Zhang et al.28 Chemicals, peptides, and recombinant proteins Forskolin Tocris Cat# 1099 CNQX disodium salt Tocris Cat# 1045 D-AP5 Tocris Cat# 0106 Tetrodotoxin Tocris Cat# 1078 ZD7288 Tocris Cat# 1000 Myristoylated PKI Tocris Cat# 2546 H-89 dihydrochloride Tocris Cat# 2910 L-cis-Diltiazem hydrochloride Tocris Cat# 0685 Experimental models: Organisms/strains Mouse: Vglut2-ires-cre; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:016963 Software and algorithms MATLAB R2022 Mathworks https://www.mathworks.com/products/ %20matlab.htmlwww.mathworks.com/ products/ matlab.html; RRID: SCR_001622 Scanbox Neurolabware https://scanbox.org Fiji NIH Schindelin et al.60 Other Two-photon microscope Neurolabware Cat# TL10X-2P InSight X3 laser Spectra-Physics InSight X3 16x, 0.8 NA objective Nikon CFI75 4X, 0.28 NA objective Olympus XFLUOR ..
Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Recombinant:Article Title: Transient cAMP production drives rapid and sustained spiking in brainstem parabrachial neurons to suppress feeding.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV2/1.hSyn.DIO.GreenDownwardcADDis.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.EF1a.DIO.mKate2-biPAC.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.EF1a.DIO.mKate2-PDE4D3- Cat.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.hSyn.FLEX.GCaMP6s.WPRE Chen et al.59 Addgene Cat# 100845 AAV2/9.hSyn.FLEX.ExRai-AKAR2-CW3SL Richard Huganir Zhang et al.28 Chemicals, peptides, and recombinant proteins Forskolin Tocris Cat# 1099 CNQX disodium salt Tocris Cat# 1045 D-AP5 Tocris Cat# 0106 Tetrodotoxin Tocris Cat# 1078 ZD7288 Tocris Cat# 1000 Myristoylated PKI Tocris Cat# 2546 H-89 dihydrochloride Tocris Cat# 2910 L-cis-Diltiazem hydrochloride Tocris Cat# 0685 Experimental models: Organisms/strains Mouse: Vglut2-ires-cre; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:016963 Software and algorithms MATLAB R2022 Mathworks https://www.mathworks.com/products/ %20matlab.htmlwww.mathworks.com/ products/ matlab.html; RRID: SCR_001622 Scanbox Neurolabware https://scanbox.org Fiji NIH Schindelin et al.60 Other Two-photon microscope Neurolabware Cat# TL10X-2P InSight X3 laser Spectra-Physics InSight X3 16x, 0.8 NA objective Nikon CFI75 4X, 0.28 NA objective Olympus XFLUOR ..
Article Title: Developmental refinement of the active zone nanotopography and axon wiring at the somatosensory thalamus.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa 488-conjugated streptavidin Invitrogen Cat#S11223 mouse monoclonal anti-panMunc13 BD Biosciences Cat#610998 rabbit polyclonal anti-Cav2.1 Synaptic Systems Cat#152 203 mouse monoclonal anti-Cav2.2 Synaptic Systems Cat#152 311 mouse monoclonal anti-PSD95 NeuroMab Cat#75-028 goat anti-mouse IgG1 Jackson ImmunoResearch Cat#115-005-205 donkey anti-rabbit IgG Jackson ImmunoResearch Cat#711-005-152 goat anti-mouse IgG2b Jackson ImmunoResearch Cat#115-005-207 goat anti-mouse IgG2a Jackson ImmunoResearch Cat#115-005-206 Alexa 488 NHS Thermo Fisher Scientific Cat#A20000 Alexa 647 NHS Thermo Fisher Scientific Cat#A20006 Chemicals, peptides, and recombinant proteins ( )-bicuculline methochloride Tocris Cat#0131 TEA-Cl Merck Cat#T2265 D-AP5 Tocris Cat0106 CTZ Tocris Cat#0713 QX-314 Merck Cat#L5783 Neurobiotin Vector Laboratories Cat#SP-1120 ProLongTM Glass Antifade Mountant Thermo Fisher Scientific Cat#P36980 SlowFade Diamond antifade mountant Thermo Fisher Scientific Cat#S36972 Experimental models: Organisms/strains Mouse:Krox20-Cre Patrick Charnay, Jackson Laboratory Cat# 025744 Mouse:Ai34D (B6; 129S-Gt(ROSA) 26Sortm34.1(CAG-Syp/tdTomato)Hze/J) Hongkui Zeng, Jackson Laboratory Cat# 012570 Software and algorithms IgorPro Wavemetrics https://www.wavemetrics.com/ Additional custom IgorPro scripts This work https://doi.org/10.5281/zenodo.13377591 Solis Andor https://andor.oxinst.com/ PatchMaster HEKA https://www.heka.com/ Excel Microsoft https://www.microsoft.com/ BZ-X800 analyzer Keyence https://www.keyence.com/ Metamorph Molecular Devices https://www.moleculardevices.com/ LAS-X Leica https://www.leica-microsystems.com/ ImageJ NIH, Open source https://imagej.net/ij/ Mathematica Wolfram Research https://www.wolfram.com/mathematica/ D3D SilverLabUCL https://github.com/SilverLabUCL/D3D/blob/ master/src/ucl/silver/d3d/init/InitFD_Demo.java ..
Article Title: Genetically encoded fluorescent reporters to visualize α-synuclein pathology in live brain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-EGFP Abcam Cat# ab290; RRID: AB_303395 Chicken polyclonal anti-EGFP Abcam Cat# ab13970; RRID: AB_300798 Rabbit polyclonal anti-mCherry Abcam Cat# ab167453; RRID: AB_2571870 Chicken polyclonal anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit polyclonal anti-TH Merck Millipore Cat # AB152; RRID: AB_390204 Rabbit polyclonal anti-ChAT Sigma-Aldrich Cat# AB144P; RRID: AB_2079751 Rabbit polyclonal anti-Synaptophysin ThermoFisher Cat# PA1-1043; RRID: AB_2199026 Mouse monoclonal anti-MAP2 Merck Millipore Cat# MAB3418; RRID: AB_94856 Rabbit polyclonal anti-Glutamate Sigma-Aldrich Cat# G6642; RRID: AB_259946 Rabbit polyclonal anti-p-α-Syn Abcam Cat# ab51253; RRID: AB_869973 Rabbit polyclonal anti-p62 Abcam Cat# ab91526; RRID: AB_2050336 Rabbit polyclonal anti-ubiquitin Abcam Cat# ab19247; RRID: AB_444805 Mouse monoclonal anti-LAMP1 DSHB Cat# 1D4B; RRID: AB_2134500 Mouse monoclonal anti-NeuN Merck Millipore Cat# MAB377; RRID: AB_2298772 Chemicals, peptides, and recombinant proteins Human α-Syn Generated in this study N/A Mouse α-Syn Generated in this study N/A Mouse α-Syn-6H-EGFP Generated in this study N/A Mouse α-Syn-6H-tdT Generated in this study N/A Thioflavin S Sigma Cat# T1892 Thioflavin T Sigma Cat# T3516 4-Aminopyridine (4-AP) Sigma Cat# 275875 4-amino-2-chloropyridine (4-AP-Cl) Sigma Cat# 522937 TTX Tocris Cat# 1078 D-AP5 Tocris Cat# 0106 CNQX Tocris Cat# 0190 Picrotoxin Tocris Cat# 1128 Neurobiotin Vector Laboratories Cat# SP1120 Streptavidin− Cy3 Sigma-Aldrich Cat# S6402 Triton X-100 Sigma Cat# T9284 DAPI Sigma Cat# D8417 Cal-520 AM AAT Bioquest Cat# 21130 Carmine Solarbio Cat# C8540 Activated charcoal powder Sigma Cat# 05105 Deposited data Transcriptomic data Generated in this study Deposited at Gene Expression Omnibus with the accession number GEO: GSE282108 https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282108 Mass spectrometric data Generated in this study Deposited in the MassIVE database with the accession number MSV000096430 https://massive.ucsd.edu/ProteoSAFe/ dataset.jsp?task=3f843b1dba704839978daf98e7baf0ee (Continued on next page) ll OPEN ACCESS Cell 189, 1–20.e1–e9, April 2, 2026 e1 Please cite this article in press as: Zhang et al., Genetically encoded fluorescent reporters to visualize α-synuclein pathology in live brain, Cell (2026), https://doi.org/10.1016/j.cell.2026.01.035 Resource ..
Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Article Title: The atypical adhesion GPCR ADGRA1 controls hippocampal inhibitory circuit function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Bovine Serum Albumin Fraction V Roche Cat#10735086001 Normal Goat Serum Jackson Immunoresearch Cat#005000121 Bovine Serum Albumin Sigma Cat#A3803 DAPI Roche Cat#10236276001 DMEM Gibco Cat#11995065 Fetal Bovine Serum Gibco Cat#16000044 Hanks’ Balanced Salt Solution Gibco Cat#14175095 HEPES Sigma Cat#H3375 Matrigel Membrane Matrix ThermoFisher Cat#CB-40234 Poly-D-lysine Gibco Cat#A38904-01 MEM Non-essential Amino Acid Solution Sigma Cat#M7145 Papain Worthington Biochemical Corporation Cat#LS003126 Opti-MEM Gibco Cat#31985070 Paraformaldehyde Electron Microscopy Science Cat#15714 Penicillin/Streptomycin Corning Cat#MT30002Cl B-27 supplement Gibco Cat#17504044 Neurobasal A Gibco Cat#10888022 GlutaMAX Supplement Gibco Cat#35050061 TransIT-2020 Mirus Cat#MIR5400 Cytosine β-D-arabinofuranoside Sigma Cat#C6645 Versene Gibco Cat#15040066 FuGENE6 Promega Cat#E2691 Heparin sodium salt Sigma Cat#H3393 OCT embedding compound Fisher Cat23730571 D-AP5 Tocris Cat#0106 CNQX disodium salt Tocris Cat#1045 Tetrodotoxin citrate Tocris Cat#1069 Picrotoxin Tocris Cat#1128 Adenosine 5′-Triphosphate magnesium Sigma Cat#A9187 Guanosine 5′-Triphosphate sodium Sigma Cat#G8877 Phosphocreatine disodium hydrate Sigma Cat#P7936 Cesium methanesulfonate Sigma Cat#C1426 Cesium chloride Sigma Cat#289329 Biocytin Sigma Cat#B4261 Streptavidin Alexa 647 conjugate ThermoFisher Cat#S21374 QX-314 bromide Tocris Cat#1014 Spermine Sigma Cat#S4264 Superscript IV Reverse Transcriptase Invitrogen Cat#18091050 In-Fusion Assembly system Takara Cat#638948 PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25742 Coelenterazine-400a NanoLight Technologies Cat#340 Prolong Gold Antifade Reagent Invitrogen Cat#P36930 Experimental models: Cell lines HEK293T ATCC CRL-11268 GKO HEK293 Kind gift from Asuka Inoue, Tokyo University N/A (Continued on next page) Cell Reports 45, 117255, April 28, 2026 15 ..
Microscopy:Article Title: Transient cAMP production drives rapid and sustained spiking in brainstem parabrachial neurons to suppress feeding.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains AAV2/1.hSyn.DIO.GreenDownwardcADDis.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.EF1a.DIO.mKate2-biPAC.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.EF1a.DIO.mKate2-PDE4D3- Cat.WPRE Boston Children’s Hospital Viral Core Zhang et al.28 AAV2/1.hSyn.FLEX.GCaMP6s.WPRE Chen et al.59 Addgene Cat# 100845 AAV2/9.hSyn.FLEX.ExRai-AKAR2-CW3SL Richard Huganir Zhang et al.28 Chemicals, peptides, and recombinant proteins Forskolin Tocris Cat# 1099 CNQX disodium salt Tocris Cat# 1045 D-AP5 Tocris Cat# 0106 Tetrodotoxin Tocris Cat# 1078 ZD7288 Tocris Cat# 1000 Myristoylated PKI Tocris Cat# 2546 H-89 dihydrochloride Tocris Cat# 2910 L-cis-Diltiazem hydrochloride Tocris Cat# 0685 Experimental models: Organisms/strains Mouse: Vglut2-ires-cre; C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:016963 Software and algorithms MATLAB R2022 Mathworks https://www.mathworks.com/products/ %20matlab.htmlwww.mathworks.com/ products/ matlab.html; RRID: SCR_001622 Scanbox Neurolabware https://scanbox.org Fiji NIH Schindelin et al.60 Other Two-photon microscope Neurolabware Cat# TL10X-2P InSight X3 laser Spectra-Physics InSight X3 16x, 0.8 NA objective Nikon CFI75 4X, 0.28 NA objective Olympus XFLUOR ..
Software:Article Title: Developmental refinement of the active zone nanotopography and axon wiring at the somatosensory thalamus.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa 488-conjugated streptavidin Invitrogen Cat#S11223 mouse monoclonal anti-panMunc13 BD Biosciences Cat#610998 rabbit polyclonal anti-Cav2.1 Synaptic Systems Cat#152 203 mouse monoclonal anti-Cav2.2 Synaptic Systems Cat#152 311 mouse monoclonal anti-PSD95 NeuroMab Cat#75-028 goat anti-mouse IgG1 Jackson ImmunoResearch Cat#115-005-205 donkey anti-rabbit IgG Jackson ImmunoResearch Cat#711-005-152 goat anti-mouse IgG2b Jackson ImmunoResearch Cat#115-005-207 goat anti-mouse IgG2a Jackson ImmunoResearch Cat#115-005-206 Alexa 488 NHS Thermo Fisher Scientific Cat#A20000 Alexa 647 NHS Thermo Fisher Scientific Cat#A20006 Chemicals, peptides, and recombinant proteins ( )-bicuculline methochloride Tocris Cat#0131 TEA-Cl Merck Cat#T2265 D-AP5 Tocris Cat0106 CTZ Tocris Cat#0713 QX-314 Merck Cat#L5783 Neurobiotin Vector Laboratories Cat#SP-1120 ProLongTM Glass Antifade Mountant Thermo Fisher Scientific Cat#P36980 SlowFade Diamond antifade mountant Thermo Fisher Scientific Cat#S36972 Experimental models: Organisms/strains Mouse:Krox20-Cre Patrick Charnay, Jackson Laboratory Cat# 025744 Mouse:Ai34D (B6; 129S-Gt(ROSA) 26Sortm34.1(CAG-Syp/tdTomato)Hze/J) Hongkui Zeng, Jackson Laboratory Cat# 012570 Software and algorithms IgorPro Wavemetrics https://www.wavemetrics.com/ Additional custom IgorPro scripts This work https://doi.org/10.5281/zenodo.13377591 Solis Andor https://andor.oxinst.com/ PatchMaster HEKA https://www.heka.com/ Excel Microsoft https://www.microsoft.com/ BZ-X800 analyzer Keyence https://www.keyence.com/ Metamorph Molecular Devices https://www.moleculardevices.com/ LAS-X Leica https://www.leica-microsystems.com/ ImageJ NIH, Open source https://imagej.net/ij/ Mathematica Wolfram Research https://www.wolfram.com/mathematica/ D3D SilverLabUCL https://github.com/SilverLabUCL/D3D/blob/ master/src/ucl/silver/d3d/init/InitFD_Demo.java ..
Article Title: Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development.
Article Snippet: Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015). .. The Neuro2a mouse neuroblastoma line (CCL-131) was obtained from ATCC and was cultured in MEM (Gibco; Cat. #11090-81 or Corning; Cat. #10-010-CM) supplemented with 10% Reagent/resource Reference or Source Identifier or Catalog Number NMDA TOCRIS Cat#0114 D-AP5 TOCRIS Cat0106 KN62 TOCRIS Cat#1277 CNQX TOCRIS Cat#0190 Software ImageJ Schneider et al, 2012 https://imagej.nih.gov/ij/ Imaris 9.3.1 (Bitplane) Oxford Instruments https://imaris.oxinst.com ezTrack Pennington et al, 2019 https://github.com/denisecailab/ezTrack Other HisTrap column GE Healthcare Cat#17-5247-01 Glutathione Sepharose 4B GE Healthcare Cat#17-0756-01 Protein G agarose Roche 11243233001 square-wave electroporation generator BTX Inc. model ECM 830 carboxylate-modified FluoSpheresTM microspheres (0.2 μm, blue fluorescing (365/415) Thermo Fisher Scientific Cat#F8805 BaseScopeTM Duplex Detection Reagent Kit ACDBio Cat#323800 Mendeley DATA Mendeley data Reserved DOI: 10.17632/9mckw4t9f3.2 Raw and analyzed data of RNA-seq Gene Expression Omnibus https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE288599 Username:reviewer_pxd060131@ebi.ac.uk Password:cR9FHFPH0RrP Protein interaction IP-MS data PRIDE Project accession: PXD060131 Token:cR9FHFPH0RrP Behavior dataset BioImage Archive https://www.ebi.ac.uk/biostudies/studies/SBIAD2140 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 202.51.89.179. .. Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015).
Generated:Article Title: Genetically encoded fluorescent reporters to visualize α-synuclein pathology in live brain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-EGFP Abcam Cat# ab290; RRID: AB_303395 Chicken polyclonal anti-EGFP Abcam Cat# ab13970; RRID: AB_300798 Rabbit polyclonal anti-mCherry Abcam Cat# ab167453; RRID: AB_2571870 Chicken polyclonal anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit polyclonal anti-TH Merck Millipore Cat # AB152; RRID: AB_390204 Rabbit polyclonal anti-ChAT Sigma-Aldrich Cat# AB144P; RRID: AB_2079751 Rabbit polyclonal anti-Synaptophysin ThermoFisher Cat# PA1-1043; RRID: AB_2199026 Mouse monoclonal anti-MAP2 Merck Millipore Cat# MAB3418; RRID: AB_94856 Rabbit polyclonal anti-Glutamate Sigma-Aldrich Cat# G6642; RRID: AB_259946 Rabbit polyclonal anti-p-α-Syn Abcam Cat# ab51253; RRID: AB_869973 Rabbit polyclonal anti-p62 Abcam Cat# ab91526; RRID: AB_2050336 Rabbit polyclonal anti-ubiquitin Abcam Cat# ab19247; RRID: AB_444805 Mouse monoclonal anti-LAMP1 DSHB Cat# 1D4B; RRID: AB_2134500 Mouse monoclonal anti-NeuN Merck Millipore Cat# MAB377; RRID: AB_2298772 Chemicals, peptides, and recombinant proteins Human α-Syn Generated in this study N/A Mouse α-Syn Generated in this study N/A Mouse α-Syn-6H-EGFP Generated in this study N/A Mouse α-Syn-6H-tdT Generated in this study N/A Thioflavin S Sigma Cat# T1892 Thioflavin T Sigma Cat# T3516 4-Aminopyridine (4-AP) Sigma Cat# 275875 4-amino-2-chloropyridine (4-AP-Cl) Sigma Cat# 522937 TTX Tocris Cat# 1078 D-AP5 Tocris Cat# 0106 CNQX Tocris Cat# 0190 Picrotoxin Tocris Cat# 1128 Neurobiotin Vector Laboratories Cat# SP1120 Streptavidin− Cy3 Sigma-Aldrich Cat# S6402 Triton X-100 Sigma Cat# T9284 DAPI Sigma Cat# D8417 Cal-520 AM AAT Bioquest Cat# 21130 Carmine Solarbio Cat# C8540 Activated charcoal powder Sigma Cat# 05105 Deposited data Transcriptomic data Generated in this study Deposited at Gene Expression Omnibus with the accession number GEO: GSE282108 https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282108 Mass spectrometric data Generated in this study Deposited in the MassIVE database with the accession number MSV000096430 https://massive.ucsd.edu/ProteoSAFe/ dataset.jsp?task=3f843b1dba704839978daf98e7baf0ee (Continued on next page) ll OPEN ACCESS Cell 189, 1–20.e1–e9, April 2, 2026 e1 Please cite this article in press as: Zhang et al., Genetically encoded fluorescent reporters to visualize α-synuclein pathology in live brain, Cell (2026), https://doi.org/10.1016/j.cell.2026.01.035 Resource ..
Gene Expression:Article Title: Genetically encoded fluorescent reporters to visualize α-synuclein pathology in live brain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-EGFP Abcam Cat# ab290; RRID: AB_303395 Chicken polyclonal anti-EGFP Abcam Cat# ab13970; RRID: AB_300798 Rabbit polyclonal anti-mCherry Abcam Cat# ab167453; RRID: AB_2571870 Chicken polyclonal anti-mCherry Abcam Cat# ab205402; RRID: AB_2722769 Rabbit polyclonal anti-TH Merck Millipore Cat # AB152; RRID: AB_390204 Rabbit polyclonal anti-ChAT Sigma-Aldrich Cat# AB144P; RRID: AB_2079751 Rabbit polyclonal anti-Synaptophysin ThermoFisher Cat# PA1-1043; RRID: AB_2199026 Mouse monoclonal anti-MAP2 Merck Millipore Cat# MAB3418; RRID: AB_94856 Rabbit polyclonal anti-Glutamate Sigma-Aldrich Cat# G6642; RRID: AB_259946 Rabbit polyclonal anti-p-α-Syn Abcam Cat# ab51253; RRID: AB_869973 Rabbit polyclonal anti-p62 Abcam Cat# ab91526; RRID: AB_2050336 Rabbit polyclonal anti-ubiquitin Abcam Cat# ab19247; RRID: AB_444805 Mouse monoclonal anti-LAMP1 DSHB Cat# 1D4B; RRID: AB_2134500 Mouse monoclonal anti-NeuN Merck Millipore Cat# MAB377; RRID: AB_2298772 Chemicals, peptides, and recombinant proteins Human α-Syn Generated in this study N/A Mouse α-Syn Generated in this study N/A Mouse α-Syn-6H-EGFP Generated in this study N/A Mouse α-Syn-6H-tdT Generated in this study N/A Thioflavin S Sigma Cat# T1892 Thioflavin T Sigma Cat# T3516 4-Aminopyridine (4-AP) Sigma Cat# 275875 4-amino-2-chloropyridine (4-AP-Cl) Sigma Cat# 522937 TTX Tocris Cat# 1078 D-AP5 Tocris Cat# 0106 CNQX Tocris Cat# 0190 Picrotoxin Tocris Cat# 1128 Neurobiotin Vector Laboratories Cat# SP1120 Streptavidin− Cy3 Sigma-Aldrich Cat# S6402 Triton X-100 Sigma Cat# T9284 DAPI Sigma Cat# D8417 Cal-520 AM AAT Bioquest Cat# 21130 Carmine Solarbio Cat# C8540 Activated charcoal powder Sigma Cat# 05105 Deposited data Transcriptomic data Generated in this study Deposited at Gene Expression Omnibus with the accession number GEO: GSE282108 https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE282108 Mass spectrometric data Generated in this study Deposited in the MassIVE database with the accession number MSV000096430 https://massive.ucsd.edu/ProteoSAFe/ dataset.jsp?task=3f843b1dba704839978daf98e7baf0ee (Continued on next page) ll OPEN ACCESS Cell 189, 1–20.e1–e9, April 2, 2026 e1 Please cite this article in press as: Zhang et al., Genetically encoded fluorescent reporters to visualize α-synuclein pathology in live brain, Cell (2026), https://doi.org/10.1016/j.cell.2026.01.035 Resource ..
Article Title: Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development.
Article Snippet: Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015). .. The Neuro2a mouse neuroblastoma line (CCL-131) was obtained from ATCC and was cultured in MEM (Gibco; Cat. #11090-81 or Corning; Cat. #10-010-CM) supplemented with 10% Reagent/resource Reference or Source Identifier or Catalog Number NMDA TOCRIS Cat#0114 D-AP5 TOCRIS Cat0106 KN62 TOCRIS Cat#1277 CNQX TOCRIS Cat#0190 Software ImageJ Schneider et al, 2012 https://imagej.nih.gov/ij/ Imaris 9.3.1 (Bitplane) Oxford Instruments https://imaris.oxinst.com ezTrack Pennington et al, 2019 https://github.com/denisecailab/ezTrack Other HisTrap column GE Healthcare Cat#17-5247-01 Glutathione Sepharose 4B GE Healthcare Cat#17-0756-01 Protein G agarose Roche 11243233001 square-wave electroporation generator BTX Inc. model ECM 830 carboxylate-modified FluoSpheresTM microspheres (0.2 μm, blue fluorescing (365/415) Thermo Fisher Scientific Cat#F8805 BaseScopeTM Duplex Detection Reagent Kit ACDBio Cat#323800 Mendeley DATA Mendeley data Reserved DOI: 10.17632/9mckw4t9f3.2 Raw and analyzed data of RNA-seq Gene Expression Omnibus https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE288599 Username:reviewer_pxd060131@ebi.ac.uk Password:cR9FHFPH0RrP Protein interaction IP-MS data PRIDE Project accession: PXD060131 Token:cR9FHFPH0RrP Behavior dataset BioImage Archive https://www.ebi.ac.uk/biostudies/studies/SBIAD2140 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 202.51.89.179. .. Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015).
Cell Culture:Article Title: Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development.
Article Snippet: Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015). .. The Neuro2a mouse neuroblastoma line (CCL-131) was obtained from ATCC and was cultured in MEM (Gibco; Cat. #11090-81 or Corning; Cat. #10-010-CM) supplemented with 10% Reagent/resource Reference or Source Identifier or Catalog Number NMDA TOCRIS Cat#0114 D-AP5 TOCRIS Cat0106 KN62 TOCRIS Cat#1277 CNQX TOCRIS Cat#0190 Software ImageJ Schneider et al, 2012 https://imagej.nih.gov/ij/ Imaris 9.3.1 (Bitplane) Oxford Instruments https://imaris.oxinst.com ezTrack Pennington et al, 2019 https://github.com/denisecailab/ezTrack Other HisTrap column GE Healthcare Cat#17-5247-01 Glutathione Sepharose 4B GE Healthcare Cat#17-0756-01 Protein G agarose Roche 11243233001 square-wave electroporation generator BTX Inc. model ECM 830 carboxylate-modified FluoSpheresTM microspheres (0.2 μm, blue fluorescing (365/415) Thermo Fisher Scientific Cat#F8805 BaseScopeTM Duplex Detection Reagent Kit ACDBio Cat#323800 Mendeley DATA Mendeley data Reserved DOI: 10.17632/9mckw4t9f3.2 Raw and analyzed data of RNA-seq Gene Expression Omnibus https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE288599 Username:reviewer_pxd060131@ebi.ac.uk Password:cR9FHFPH0RrP Protein interaction IP-MS data PRIDE Project accession: PXD060131 Token:cR9FHFPH0RrP Behavior dataset BioImage Archive https://www.ebi.ac.uk/biostudies/studies/SBIAD2140 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 202.51.89.179. .. Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015).
Electroporation:Article Title: Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development.
Article Snippet: Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015). .. The Neuro2a mouse neuroblastoma line (CCL-131) was obtained from ATCC and was cultured in MEM (Gibco; Cat. #11090-81 or Corning; Cat. #10-010-CM) supplemented with 10% Reagent/resource Reference or Source Identifier or Catalog Number NMDA TOCRIS Cat#0114 D-AP5 TOCRIS Cat0106 KN62 TOCRIS Cat#1277 CNQX TOCRIS Cat#0190 Software ImageJ Schneider et al, 2012 https://imagej.nih.gov/ij/ Imaris 9.3.1 (Bitplane) Oxford Instruments https://imaris.oxinst.com ezTrack Pennington et al, 2019 https://github.com/denisecailab/ezTrack Other HisTrap column GE Healthcare Cat#17-5247-01 Glutathione Sepharose 4B GE Healthcare Cat#17-0756-01 Protein G agarose Roche 11243233001 square-wave electroporation generator BTX Inc. model ECM 830 carboxylate-modified FluoSpheresTM microspheres (0.2 μm, blue fluorescing (365/415) Thermo Fisher Scientific Cat#F8805 BaseScopeTM Duplex Detection Reagent Kit ACDBio Cat#323800 Mendeley DATA Mendeley data Reserved DOI: 10.17632/9mckw4t9f3.2 Raw and analyzed data of RNA-seq Gene Expression Omnibus https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE288599 Username:reviewer_pxd060131@ebi.ac.uk Password:cR9FHFPH0RrP Protein interaction IP-MS data PRIDE Project accession: PXD060131 Token:cR9FHFPH0RrP Behavior dataset BioImage Archive https://www.ebi.ac.uk/biostudies/studies/SBIAD2140 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 202.51.89.179. .. Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015).
RNA Sequencing:Article Title: Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development.
Article Snippet: Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015). .. The Neuro2a mouse neuroblastoma line (CCL-131) was obtained from ATCC and was cultured in MEM (Gibco; Cat. #11090-81 or Corning; Cat. #10-010-CM) supplemented with 10% Reagent/resource Reference or Source Identifier or Catalog Number NMDA TOCRIS Cat#0114 D-AP5 TOCRIS Cat0106 KN62 TOCRIS Cat#1277 CNQX TOCRIS Cat#0190 Software ImageJ Schneider et al, 2012 https://imagej.nih.gov/ij/ Imaris 9.3.1 (Bitplane) Oxford Instruments https://imaris.oxinst.com ezTrack Pennington et al, 2019 https://github.com/denisecailab/ezTrack Other HisTrap column GE Healthcare Cat#17-5247-01 Glutathione Sepharose 4B GE Healthcare Cat#17-0756-01 Protein G agarose Roche 11243233001 square-wave electroporation generator BTX Inc. model ECM 830 carboxylate-modified FluoSpheresTM microspheres (0.2 μm, blue fluorescing (365/415) Thermo Fisher Scientific Cat#F8805 BaseScopeTM Duplex Detection Reagent Kit ACDBio Cat#323800 Mendeley DATA Mendeley data Reserved DOI: 10.17632/9mckw4t9f3.2 Raw and analyzed data of RNA-seq Gene Expression Omnibus https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE288599 Username:reviewer_pxd060131@ebi.ac.uk Password:cR9FHFPH0RrP Protein interaction IP-MS data PRIDE Project accession: PXD060131 Token:cR9FHFPH0RrP Behavior dataset BioImage Archive https://www.ebi.ac.uk/biostudies/studies/SBIAD2140 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 202.51.89.179. .. Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015).
Protein-Protein interactions:Article Title: Nuclear paxillin functions as a molecular switch for alternative splicing in neurons during a critical period of brain development.
Article Snippet: Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015). .. The Neuro2a mouse neuroblastoma line (CCL-131) was obtained from ATCC and was cultured in MEM (Gibco; Cat. #11090-81 or Corning; Cat. #10-010-CM) supplemented with 10% Reagent/resource Reference or Source Identifier or Catalog Number NMDA TOCRIS Cat#0114 D-AP5 TOCRIS Cat0106 KN62 TOCRIS Cat#1277 CNQX TOCRIS Cat#0190 Software ImageJ Schneider et al, 2012 https://imagej.nih.gov/ij/ Imaris 9.3.1 (Bitplane) Oxford Instruments https://imaris.oxinst.com ezTrack Pennington et al, 2019 https://github.com/denisecailab/ezTrack Other HisTrap column GE Healthcare Cat#17-5247-01 Glutathione Sepharose 4B GE Healthcare Cat#17-0756-01 Protein G agarose Roche 11243233001 square-wave electroporation generator BTX Inc. model ECM 830 carboxylate-modified FluoSpheresTM microspheres (0.2 μm, blue fluorescing (365/415) Thermo Fisher Scientific Cat#F8805 BaseScopeTM Duplex Detection Reagent Kit ACDBio Cat#323800 Mendeley DATA Mendeley data Reserved DOI: 10.17632/9mckw4t9f3.2 Raw and analyzed data of RNA-seq Gene Expression Omnibus https://www.ncbi.nlm.nih.gov/geo/query/ acc.cgi?acc=GSE288599 Username:reviewer_pxd060131@ebi.ac.uk Password:cR9FHFPH0RrP Protein interaction IP-MS data PRIDE Project accession: PXD060131 Token:cR9FHFPH0RrP Behavior dataset BioImage Archive https://www.ebi.ac.uk/biostudies/studies/SBIAD2140 © The Author(s) The EMBO Journal 17 D ow nloaded from https://w w w .em bopress.org on O ctober 16, 2025 from IP 202.51.89.179. .. Primary cortical neurons were prepared from rat embryos at embryonic day (E) E18 and cultured in Neurobasal medium (Gibco; Cat. #21103049) supplemented with Gem21 NeuroPlex (Gemini Bio-Products; Cat. #400-160-010) (Dotti et al, 1988; Hsu et al, 2015).
Plasmid Preparation:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Bioprocessing:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Enzyme-linked Immunosorbent Assay:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Reverse Transcription:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Article Title: The atypical adhesion GPCR ADGRA1 controls hippocampal inhibitory circuit function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Bovine Serum Albumin Fraction V Roche Cat#10735086001 Normal Goat Serum Jackson Immunoresearch Cat#005000121 Bovine Serum Albumin Sigma Cat#A3803 DAPI Roche Cat#10236276001 DMEM Gibco Cat#11995065 Fetal Bovine Serum Gibco Cat#16000044 Hanks’ Balanced Salt Solution Gibco Cat#14175095 HEPES Sigma Cat#H3375 Matrigel Membrane Matrix ThermoFisher Cat#CB-40234 Poly-D-lysine Gibco Cat#A38904-01 MEM Non-essential Amino Acid Solution Sigma Cat#M7145 Papain Worthington Biochemical Corporation Cat#LS003126 Opti-MEM Gibco Cat#31985070 Paraformaldehyde Electron Microscopy Science Cat#15714 Penicillin/Streptomycin Corning Cat#MT30002Cl B-27 supplement Gibco Cat#17504044 Neurobasal A Gibco Cat#10888022 GlutaMAX Supplement Gibco Cat#35050061 TransIT-2020 Mirus Cat#MIR5400 Cytosine β-D-arabinofuranoside Sigma Cat#C6645 Versene Gibco Cat#15040066 FuGENE6 Promega Cat#E2691 Heparin sodium salt Sigma Cat#H3393 OCT embedding compound Fisher Cat23730571 D-AP5 Tocris Cat#0106 CNQX disodium salt Tocris Cat#1045 Tetrodotoxin citrate Tocris Cat#1069 Picrotoxin Tocris Cat#1128 Adenosine 5′-Triphosphate magnesium Sigma Cat#A9187 Guanosine 5′-Triphosphate sodium Sigma Cat#G8877 Phosphocreatine disodium hydrate Sigma Cat#P7936 Cesium methanesulfonate Sigma Cat#C1426 Cesium chloride Sigma Cat#289329 Biocytin Sigma Cat#B4261 Streptavidin Alexa 647 conjugate ThermoFisher Cat#S21374 QX-314 bromide Tocris Cat#1014 Spermine Sigma Cat#S4264 Superscript IV Reverse Transcriptase Invitrogen Cat#18091050 In-Fusion Assembly system Takara Cat#638948 PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25742 Coelenterazine-400a NanoLight Technologies Cat#340 Prolong Gold Antifade Reagent Invitrogen Cat#P36930 Experimental models: Cell lines HEK293T ATCC CRL-11268 GKO HEK293 Kind gift from Asuka Inoue, Tokyo University N/A (Continued on next page) Cell Reports 45, 117255, April 28, 2026 15 ..
SYBR Green Assay:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Article Title: The atypical adhesion GPCR ADGRA1 controls hippocampal inhibitory circuit function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Bovine Serum Albumin Fraction V Roche Cat#10735086001 Normal Goat Serum Jackson Immunoresearch Cat#005000121 Bovine Serum Albumin Sigma Cat#A3803 DAPI Roche Cat#10236276001 DMEM Gibco Cat#11995065 Fetal Bovine Serum Gibco Cat#16000044 Hanks’ Balanced Salt Solution Gibco Cat#14175095 HEPES Sigma Cat#H3375 Matrigel Membrane Matrix ThermoFisher Cat#CB-40234 Poly-D-lysine Gibco Cat#A38904-01 MEM Non-essential Amino Acid Solution Sigma Cat#M7145 Papain Worthington Biochemical Corporation Cat#LS003126 Opti-MEM Gibco Cat#31985070 Paraformaldehyde Electron Microscopy Science Cat#15714 Penicillin/Streptomycin Corning Cat#MT30002Cl B-27 supplement Gibco Cat#17504044 Neurobasal A Gibco Cat#10888022 GlutaMAX Supplement Gibco Cat#35050061 TransIT-2020 Mirus Cat#MIR5400 Cytosine β-D-arabinofuranoside Sigma Cat#C6645 Versene Gibco Cat#15040066 FuGENE6 Promega Cat#E2691 Heparin sodium salt Sigma Cat#H3393 OCT embedding compound Fisher Cat23730571 D-AP5 Tocris Cat#0106 CNQX disodium salt Tocris Cat#1045 Tetrodotoxin citrate Tocris Cat#1069 Picrotoxin Tocris Cat#1128 Adenosine 5′-Triphosphate magnesium Sigma Cat#A9187 Guanosine 5′-Triphosphate sodium Sigma Cat#G8877 Phosphocreatine disodium hydrate Sigma Cat#P7936 Cesium methanesulfonate Sigma Cat#C1426 Cesium chloride Sigma Cat#289329 Biocytin Sigma Cat#B4261 Streptavidin Alexa 647 conjugate ThermoFisher Cat#S21374 QX-314 bromide Tocris Cat#1014 Spermine Sigma Cat#S4264 Superscript IV Reverse Transcriptase Invitrogen Cat#18091050 In-Fusion Assembly system Takara Cat#638948 PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25742 Coelenterazine-400a NanoLight Technologies Cat#340 Prolong Gold Antifade Reagent Invitrogen Cat#P36930 Experimental models: Cell lines HEK293T ATCC CRL-11268 GKO HEK293 Kind gift from Asuka Inoue, Tokyo University N/A (Continued on next page) Cell Reports 45, 117255, April 28, 2026 15 ..
RNAscope:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Positive Control:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Negative Control:Article Title: Pharmacological administration of FGF21 reverses obesity through a parabrachial-projecting neuron population in the hindbrain.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies HA-Tag (C29F4) Rabbit Monoclonal Antibody Cell signaling 3724 c-Fos (9F6) Rabbit Monoclonal Antibody Cell signaling 2250 Alexa FluorTM 568 goat anti-rabbit IgG (H + L) Invitrogen 3154584 Alexa FluorTM 488 goat anti-rabbit IgG (H + L) Invitrogen 3192622 Bacterial and virus strains AAV2/9CMVCreEGFP University of Iowa viral vector core N/A AAV2/9CMVeGFP University of Iowa viral vector core N/A AAV-hSyn-DIO-hM3Dq(Gq)-mCherry Addgene Cat# 44361-AAV2 AAV-hSyn-DIO-mCherry Addgene Cat# 50459-AAV2 AAV-hSyn-DIO-EGFP Addgene Cat# 50457-AAVrg AAV-FLEX-tdTomato Addgene Cat# 28306 AAV-EF1a-Flpo Addgene Cat# 55637-AAVrg AAV-EF1a-fDIO-Cre Addgene Cat# 121675-AAV9 Chemicals, peptides, and recombinant proteins Recombinant hFGF21 NovoNordisk N/A PF-05231023 Pfizer N/A CNQX Tocris Cat#: 0190 D-AP5 Tocris Cat#: 0106 PTX Tocris Cat#: 1128 Deschloroclozapine dihydrochloride (DCZ) (water soluble) Hello Bio HB9126 Clozapine-N-oxide Tocris Cat#: 4936 Gill’s Hematoxylin I StatLab SL97-16 PINT Ammonium hydroxide solution Sigma 320145 Critical commercial assays Fibroblast Growth Factor 21 Human ELISA BioVendor Cat#: RD191108200R Ultra Sensitive Mouse Insulin ELISA Crystal Chem 90080 Infinity Triglyceride Infinity, Thermo Scientific TR13421 Verichem Labs Matrix Plus Chemistry Reference Kit Fisher Scientific NC9592194 High-Capacity cDNA Reverse Transcription Kit Life Technologies 4368814 SYBR green SYBR Greener QPCR for ABI 2000rxns Thermofisher 1176002K Autokit Glucose Wako Diagnostics 439–90901 RNAscope 2.5 HD Detection Reagent -RED Advanced Cell Diagnostics 322360 RNAscope H2O2 and Protease Reagents Advanced Cell Diagnostics 322381 RNAscope Wash Buffer Reagents Advanced Cell Diagnostics 310091 RNAscope Target Retrieval Reagents Advanced Cell Diagnostics 322000 RNAscope Positive control probe Mm PPIB Advanced Cell Diagnostics 313911 RNAscope Negative control probe Mm DapB Advanced Cell Diagnostics 310043 RNAscope Probe - Mm-Klb-O1 Advanced Cell Diagnostics 481211 (Continued on next page) Cell Reports 45, 117093, April 28, 2026 15 .. The following mice were utilized in these studies: Nkx2.1-Cre (JAX 008661), KLBfl/fl,18 KLB loxTB,50 KLB-Cre; Vglut2-flp-Ai65,29 and KLB-Cre.31 All mice used in experiments were males on a C57BL/6J genetic background, except for KLB loxTB male mice which were on a mixed background (C57BL6/129Sv).
Membrane:Article Title: The atypical adhesion GPCR ADGRA1 controls hippocampal inhibitory circuit function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Bovine Serum Albumin Fraction V Roche Cat#10735086001 Normal Goat Serum Jackson Immunoresearch Cat#005000121 Bovine Serum Albumin Sigma Cat#A3803 DAPI Roche Cat#10236276001 DMEM Gibco Cat#11995065 Fetal Bovine Serum Gibco Cat#16000044 Hanks’ Balanced Salt Solution Gibco Cat#14175095 HEPES Sigma Cat#H3375 Matrigel Membrane Matrix ThermoFisher Cat#CB-40234 Poly-D-lysine Gibco Cat#A38904-01 MEM Non-essential Amino Acid Solution Sigma Cat#M7145 Papain Worthington Biochemical Corporation Cat#LS003126 Opti-MEM Gibco Cat#31985070 Paraformaldehyde Electron Microscopy Science Cat#15714 Penicillin/Streptomycin Corning Cat#MT30002Cl B-27 supplement Gibco Cat#17504044 Neurobasal A Gibco Cat#10888022 GlutaMAX Supplement Gibco Cat#35050061 TransIT-2020 Mirus Cat#MIR5400 Cytosine β-D-arabinofuranoside Sigma Cat#C6645 Versene Gibco Cat#15040066 FuGENE6 Promega Cat#E2691 Heparin sodium salt Sigma Cat#H3393 OCT embedding compound Fisher Cat23730571 D-AP5 Tocris Cat#0106 CNQX disodium salt Tocris Cat#1045 Tetrodotoxin citrate Tocris Cat#1069 Picrotoxin Tocris Cat#1128 Adenosine 5′-Triphosphate magnesium Sigma Cat#A9187 Guanosine 5′-Triphosphate sodium Sigma Cat#G8877 Phosphocreatine disodium hydrate Sigma Cat#P7936 Cesium methanesulfonate Sigma Cat#C1426 Cesium chloride Sigma Cat#289329 Biocytin Sigma Cat#B4261 Streptavidin Alexa 647 conjugate ThermoFisher Cat#S21374 QX-314 bromide Tocris Cat#1014 Spermine Sigma Cat#S4264 Superscript IV Reverse Transcriptase Invitrogen Cat#18091050 In-Fusion Assembly system Takara Cat#638948 PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25742 Coelenterazine-400a NanoLight Technologies Cat#340 Prolong Gold Antifade Reagent Invitrogen Cat#P36930 Experimental models: Cell lines HEK293T ATCC CRL-11268 GKO HEK293 Kind gift from Asuka Inoue, Tokyo University N/A (Continued on next page) Cell Reports 45, 117255, April 28, 2026 15 ..
Electron Microscopy:Article Title: The atypical adhesion GPCR ADGRA1 controls hippocampal inhibitory circuit function.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Bovine Serum Albumin Fraction V Roche Cat#10735086001 Normal Goat Serum Jackson Immunoresearch Cat#005000121 Bovine Serum Albumin Sigma Cat#A3803 DAPI Roche Cat#10236276001 DMEM Gibco Cat#11995065 Fetal Bovine Serum Gibco Cat#16000044 Hanks’ Balanced Salt Solution Gibco Cat#14175095 HEPES Sigma Cat#H3375 Matrigel Membrane Matrix ThermoFisher Cat#CB-40234 Poly-D-lysine Gibco Cat#A38904-01 MEM Non-essential Amino Acid Solution Sigma Cat#M7145 Papain Worthington Biochemical Corporation Cat#LS003126 Opti-MEM Gibco Cat#31985070 Paraformaldehyde Electron Microscopy Science Cat#15714 Penicillin/Streptomycin Corning Cat#MT30002Cl B-27 supplement Gibco Cat#17504044 Neurobasal A Gibco Cat#10888022 GlutaMAX Supplement Gibco Cat#35050061 TransIT-2020 Mirus Cat#MIR5400 Cytosine β-D-arabinofuranoside Sigma Cat#C6645 Versene Gibco Cat#15040066 FuGENE6 Promega Cat#E2691 Heparin sodium salt Sigma Cat#H3393 OCT embedding compound Fisher Cat23730571 D-AP5 Tocris Cat#0106 CNQX disodium salt Tocris Cat#1045 Tetrodotoxin citrate Tocris Cat#1069 Picrotoxin Tocris Cat#1128 Adenosine 5′-Triphosphate magnesium Sigma Cat#A9187 Guanosine 5′-Triphosphate sodium Sigma Cat#G8877 Phosphocreatine disodium hydrate Sigma Cat#P7936 Cesium methanesulfonate Sigma Cat#C1426 Cesium chloride Sigma Cat#289329 Biocytin Sigma Cat#B4261 Streptavidin Alexa 647 conjugate ThermoFisher Cat#S21374 QX-314 bromide Tocris Cat#1014 Spermine Sigma Cat#S4264 Superscript IV Reverse Transcriptase Invitrogen Cat#18091050 In-Fusion Assembly system Takara Cat#638948 PowerUp SYBR Green Master Mix Applied Biosystems Cat#A25742 Coelenterazine-400a NanoLight Technologies Cat#340 Prolong Gold Antifade Reagent Invitrogen Cat#P36930 Experimental models: Cell lines HEK293T ATCC CRL-11268 GKO HEK293 Kind gift from Asuka Inoue, Tokyo University N/A (Continued on next page) Cell Reports 45, 117255, April 28, 2026 15 ..
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